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tlr4 protein stability  (MedChemExpress)


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    MedChemExpress tlr4 protein stability
    Fig. 3 TRIM55 decreases the expression of <t>TLR4</t> by promoting the K48-linked ubiquitination of TLR4. A The effect of TRIM55 on the protein level and ubiquitination level of TLR4 was evaluated using immunoblotting. B The interaction between TRIM55 and TLR4 proteins was analyzed by Co-IP. C PC12 cells were transfected with Flag-TRIM55, His-TLR4, and WT/K48R/K63R HA-UB. After IP with His, immunoblotting was performed with anti-HA. D PC12 cells were transfected with HA-UB, His-TLR4, and WT TRIM55 or MUT TRIM55. After IP with His, immunoblotting was performed with anti-HA. E PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, and treated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. F PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, treated with MG132, and then stimulated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. (n=3 independent biological replicates/group)
    Tlr4 Protein Stability, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr4+protein+stability/Toll-Like+Receptor+4+Antibody/pm40420098-81-2-14
    Average 97 stars, based on 46 article reviews
    tlr4 protein stability - by Bioz Stars, 2026-09
    97/100 stars

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    1) Product Images from "TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4."

    Article Title: TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.

    Journal: Journal of orthopaedic surgery and research

    doi: 10.1186/s13018-025-05922-w

    Fig. 3 TRIM55 decreases the expression of TLR4 by promoting the K48-linked ubiquitination of TLR4. A The effect of TRIM55 on the protein level and ubiquitination level of TLR4 was evaluated using immunoblotting. B The interaction between TRIM55 and TLR4 proteins was analyzed by Co-IP. C PC12 cells were transfected with Flag-TRIM55, His-TLR4, and WT/K48R/K63R HA-UB. After IP with His, immunoblotting was performed with anti-HA. D PC12 cells were transfected with HA-UB, His-TLR4, and WT TRIM55 or MUT TRIM55. After IP with His, immunoblotting was performed with anti-HA. E PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, and treated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. F PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, treated with MG132, and then stimulated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. (n=3 independent biological replicates/group)
    Figure Legend Snippet: Fig. 3 TRIM55 decreases the expression of TLR4 by promoting the K48-linked ubiquitination of TLR4. A The effect of TRIM55 on the protein level and ubiquitination level of TLR4 was evaluated using immunoblotting. B The interaction between TRIM55 and TLR4 proteins was analyzed by Co-IP. C PC12 cells were transfected with Flag-TRIM55, His-TLR4, and WT/K48R/K63R HA-UB. After IP with His, immunoblotting was performed with anti-HA. D PC12 cells were transfected with HA-UB, His-TLR4, and WT TRIM55 or MUT TRIM55. After IP with His, immunoblotting was performed with anti-HA. E PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, and treated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. F PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, treated with MG132, and then stimulated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. (n=3 independent biological replicates/group)

    Techniques Used: Expressing, Ubiquitin Proteomics, Western Blot, Co-Immunoprecipitation Assay, Transfection, Over Expression

    Fig. 4 Overexpression of TLR4 abolishes the protective effects of TRIM55 on LPS-induced PC12 cells. A PC12 cells were transfected with TLR4 overexpression plasmids or empty vectors, and the expression of TLR4 was measured using qPCR. B-G After overexpressing TRIM55 and TLR4, PC12 cells were treated with LPS for 24 h. B The cell viability was detected with the CCK-8 assay. C-D The cell apoptosis was measured by TUNEL staining, and TUNEL-positive cells were counted. E-G The contents of IL-1β, IL-6, and TNF-α were measured by ELISA assay. All data are expressed as the means ± SD. (n=3 independent biological replicates/group)
    Figure Legend Snippet: Fig. 4 Overexpression of TLR4 abolishes the protective effects of TRIM55 on LPS-induced PC12 cells. A PC12 cells were transfected with TLR4 overexpression plasmids or empty vectors, and the expression of TLR4 was measured using qPCR. B-G After overexpressing TRIM55 and TLR4, PC12 cells were treated with LPS for 24 h. B The cell viability was detected with the CCK-8 assay. C-D The cell apoptosis was measured by TUNEL staining, and TUNEL-positive cells were counted. E-G The contents of IL-1β, IL-6, and TNF-α were measured by ELISA assay. All data are expressed as the means ± SD. (n=3 independent biological replicates/group)

    Techniques Used: Over Expression, Transfection, Expressing, CCK-8 Assay, TUNEL Assay, Staining, Enzyme-linked Immunosorbent Assay

    Related Articles

    Transfection:

    Article Title: TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4
    Article Snippet: .. To investigate TLR4 protein stability, transfected cells were first treated with MG132 (20 μM, MedChemExpress) for 8 h to suppress proteasomal activity. .. Subsequently, cells were treated with CHX (100 μg/mL, MedChemExpress, Monmouth Junction, NJ, USA) to halt ongoing protein synthesis.

    Article Title: TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.
    Article Snippet: .. To investigate TLR4 protein stability, transfected cells were first treated with MG132 (20 μM, MedChemExpress) for 8 h to suppress proteasomal activity. .. Subsequently, cells were treated with CHX (100 μg/mL, MedChemExpress, Monmouth Junction, NJ, USA) to halt ongoing protein synthesis.

    Activity Assay:

    Article Title: TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4
    Article Snippet: .. To investigate TLR4 protein stability, transfected cells were first treated with MG132 (20 μM, MedChemExpress) for 8 h to suppress proteasomal activity. .. Subsequently, cells were treated with CHX (100 μg/mL, MedChemExpress, Monmouth Junction, NJ, USA) to halt ongoing protein synthesis.

    Article Title: TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.
    Article Snippet: .. To investigate TLR4 protein stability, transfected cells were first treated with MG132 (20 μM, MedChemExpress) for 8 h to suppress proteasomal activity. .. Subsequently, cells were treated with CHX (100 μg/mL, MedChemExpress, Monmouth Junction, NJ, USA) to halt ongoing protein synthesis.



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    tlr4 protein stability  (MedChemExpress)
    97
    MedChemExpress tlr4 protein stability
    Fig. 3 TRIM55 decreases the expression of <t>TLR4</t> by promoting the K48-linked ubiquitination of TLR4. A The effect of TRIM55 on the protein level and ubiquitination level of TLR4 was evaluated using immunoblotting. B The interaction between TRIM55 and TLR4 proteins was analyzed by Co-IP. C PC12 cells were transfected with Flag-TRIM55, His-TLR4, and WT/K48R/K63R HA-UB. After IP with His, immunoblotting was performed with anti-HA. D PC12 cells were transfected with HA-UB, His-TLR4, and WT TRIM55 or MUT TRIM55. After IP with His, immunoblotting was performed with anti-HA. E PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, and treated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. F PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, treated with MG132, and then stimulated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. (n=3 independent biological replicates/group)
    Tlr4 Protein Stability, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr4+protein+stability/Toll-Like+Receptor+4+Antibody/pm40420098-81-2-14
    Average 97 stars, based on 1 article reviews
    tlr4 protein stability - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 3 TRIM55 decreases the expression of TLR4 by promoting the K48-linked ubiquitination of TLR4. A The effect of TRIM55 on the protein level and ubiquitination level of TLR4 was evaluated using immunoblotting. B The interaction between TRIM55 and TLR4 proteins was analyzed by Co-IP. C PC12 cells were transfected with Flag-TRIM55, His-TLR4, and WT/K48R/K63R HA-UB. After IP with His, immunoblotting was performed with anti-HA. D PC12 cells were transfected with HA-UB, His-TLR4, and WT TRIM55 or MUT TRIM55. After IP with His, immunoblotting was performed with anti-HA. E PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, and treated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. F PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, treated with MG132, and then stimulated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. (n=3 independent biological replicates/group)

    Journal: Journal of orthopaedic surgery and research

    Article Title: TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.

    doi: 10.1186/s13018-025-05922-w

    Figure Lengend Snippet: Fig. 3 TRIM55 decreases the expression of TLR4 by promoting the K48-linked ubiquitination of TLR4. A The effect of TRIM55 on the protein level and ubiquitination level of TLR4 was evaluated using immunoblotting. B The interaction between TRIM55 and TLR4 proteins was analyzed by Co-IP. C PC12 cells were transfected with Flag-TRIM55, His-TLR4, and WT/K48R/K63R HA-UB. After IP with His, immunoblotting was performed with anti-HA. D PC12 cells were transfected with HA-UB, His-TLR4, and WT TRIM55 or MUT TRIM55. After IP with His, immunoblotting was performed with anti-HA. E PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, and treated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. F PC12 cells were transfected with TRIM55 overexpression plasmids or empty vectors, treated with MG132, and then stimulated with CHX for 0, 6, 12, 18, and 24 h. Protein levels of TLR4 were detected using immunoblotting. (n=3 independent biological replicates/group)

    Article Snippet: To investigate TLR4 protein stability, transfected cells were first treated with MG132 (20 μM, MedChemExpress) for 8 h to suppress proteasomal activity.

    Techniques: Expressing, Ubiquitin Proteomics, Western Blot, Co-Immunoprecipitation Assay, Transfection, Over Expression

    Fig. 4 Overexpression of TLR4 abolishes the protective effects of TRIM55 on LPS-induced PC12 cells. A PC12 cells were transfected with TLR4 overexpression plasmids or empty vectors, and the expression of TLR4 was measured using qPCR. B-G After overexpressing TRIM55 and TLR4, PC12 cells were treated with LPS for 24 h. B The cell viability was detected with the CCK-8 assay. C-D The cell apoptosis was measured by TUNEL staining, and TUNEL-positive cells were counted. E-G The contents of IL-1β, IL-6, and TNF-α were measured by ELISA assay. All data are expressed as the means ± SD. (n=3 independent biological replicates/group)

    Journal: Journal of orthopaedic surgery and research

    Article Title: TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.

    doi: 10.1186/s13018-025-05922-w

    Figure Lengend Snippet: Fig. 4 Overexpression of TLR4 abolishes the protective effects of TRIM55 on LPS-induced PC12 cells. A PC12 cells were transfected with TLR4 overexpression plasmids or empty vectors, and the expression of TLR4 was measured using qPCR. B-G After overexpressing TRIM55 and TLR4, PC12 cells were treated with LPS for 24 h. B The cell viability was detected with the CCK-8 assay. C-D The cell apoptosis was measured by TUNEL staining, and TUNEL-positive cells were counted. E-G The contents of IL-1β, IL-6, and TNF-α were measured by ELISA assay. All data are expressed as the means ± SD. (n=3 independent biological replicates/group)

    Article Snippet: To investigate TLR4 protein stability, transfected cells were first treated with MG132 (20 μM, MedChemExpress) for 8 h to suppress proteasomal activity.

    Techniques: Over Expression, Transfection, Expressing, CCK-8 Assay, TUNEL Assay, Staining, Enzyme-linked Immunosorbent Assay